īocachico Prochilodus magdalenae es una especie endémica y la más importante de la pesquería continental colombiana. Species Common name Treatment a Enhancement period (h) b Reference Abramis brama Bream CPE 18–24 (Kucharczyk et al., 1997) Acipenser brevirostrum Shortnose sturgeon CPE 24 (Horváth et al., 2005) Acipenser ruthenus Sterlet CPE 24, 48, 72 /12–42 (Alavi et al., 2012 Dzyuba et al., 2012 Psenicka et al., 2008) Anguila japonica Japanese eel Salmon PE 42 days c (Kagawa et al., 2009) Brycon amazonicus Yamú CPE 24 (Velasco-Santamaría et al., 2006) Brycon insignis Tiete tetra/piabanha CPE 8 (Viveiros et al., 2012b) Brycon opalinus Pirapitinga-do-sul CPE 8 (Orfão et al., 2011) Brycon orbignyanus Piracanjuba CPE 5 ( Maria et al., 2006) Clarias gariepinus African catfish CPE, Clarias PE 24 (Viveiros et al., 2002) Colossoma macropomum Tambaqui CPE 10 (Maria et al., 2015). Homologous reFSH and reLH have been produced for zebrafish (So et al., 2005), channel catfish Table 1Representative applications of hormonal manipulations for the enhancement of spermiation using various gonadotropin (GtH) preparations of piscine origin. The semen cryopreservation protocols developed here will be used to set up a gene bank for endangered piracanjuba populations. Hatching rates of eggs fertilized with sperm cryopreserved in NaCl–yolk or BTS™ were higher than for eggs fertilized with sperm cryopreserved in M III™–yolk, but lower than control fertilizations. Motility and percentage of live spermatozoa were similar among semen cryopreserved in NaCl– yolk, M III™–yolk and BTS™, all containing 10% methylglycol, but lower than fresh control. Methylglycol was the most effective cryoprotector compared to DMSO and methanol. Egg yolk addition to the cryosolution was beneficial in samples cryopreserved in NaCl 154 mM and in M III™, but detrimental for samples cryopreserved in BTS™. There was neither beneficial nor detrimental effect of gentamycin on sperm motility at 250 μg/mL.
Motility of only 7% was observed on undiluted semen after 3 days at 4 ☌.
Piracanjuba semen diluted (1:10 total volume) in NaCl 200 mM or in Saad solution (NaCl 200 mM, Tris 30 mM) maintained motility above 35% for as long as 7 days, at 4 ☌. Post-thaw semen quality was then evaluated under three tests: sperm motility, the percentage of live spermatozoa and hatching rate (Exp. The three cryosolutions that produced the highest post-thaw sperm motility were used again to freeze semen. Sperm motility was evaluated after thawing at 60 ☌-water bath for 8 s. Semen was added to each of eighteen cryosolutions (2 yolk concentrations × 3 cryoprotectants × 3 extenders), aspirated into 0.5-mL straws, frozen in nitrogen vapor (Taylor-Wharton, CP 300, "dry shipper") and stored at − 196 ☌. Then, the effects of egg yolk (0 and 5%), cryoprotectants (dimethyl sulphoxide - DMSO, methanol, and methylglycol) and extenders (NaCl 154 mM, BTS™ Minitub and M III™ Minitub) on semen cryopreservation were evaluated (Exp. 1) or in extenders combined with the antibiotic gentamycin sulfate (Exp. Semen was diluted in some fish semen extenders (Exp. The aim of this study was to develop a protocol for semen storage of piracanjuba (Brycon orbignyanus) by both cool storage at 4 ☌ and cryopreservation at −196 ☌.